Allen Cell Imaging Collections

Tutorials

• Download and train label-free models by Greg Johnson
  
• Integrating single-cell sequencing and nuclear imaging data by Chengxiang Qiu and William Noble
  

Tools & Applications

• AICS Volume Viewer by Dan Toloudis
  
• AICSImageIO by Matthew Bowden, Jackson Brown, Jamie Sherman, Dan Toloudis
  
• Allen Cell Feature Explorer by Allen Institute for Cell Science
  
• Allen Cell Structure Segmenter by Jianxu Chen, Liya Ding, Matheus P. Viana, Melissa C. Hendershott, Ruian Yang, Irina A. Mueller, Susanne M. Rafelski
  
• Integrated Mitotic Stem Cell by Allen Institute for Cell Science
  
• Pytorch 3D Integrated Cell by Gregory R. Johnson, Rory M. Donovan-Maiye, Mary M. Maleckar
  
• Visual Guide to Human Cells by Allen Institute for Cell Science
  

Publications

• 4D hybrid model interrogates agent-level rules and parameters driving hiPS cell colony dynamics by Jessica S. Yu, Blair Lyons, Susanne Rafelski, Julie A. Theriot, Neda Bagheri & Graham T. Johnson
  
• A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes by Tanya Grancharova*, Kaytlyn A. Gerbin*, Alexander B. Rosenberg, Charles M. Roco, Joy E.Arakaki , Colette M. DeLizo, Stephanie Q. Dinh, Rory M. Donovan-Maiye, Matthew Hirano, Angelique M. Nelson, Joyce Tang, Julie A. Theriot, Calysta Yan, Vilas Menon, Sean P. Palecek, Georg Seelig & Ruwanthi N. Gunawardane
  
• A deep generative model of 3D single-cell organization by Rory M. Donovan-Maiye, Jackson M. Brown, Caleb K. Chan, Liya Ding, Calysta Yan, Nathalie Gaudreault, Julie A. Theriot, Mary M. Maleckar, Theo A. Knijnenburg & Gregory R. Johnson
  
• A human induced pluripotent stem (hiPS) cell model for the holistic study of epithelial to mesenchymal transitions (EMTs) by Caroline Hookway*, Antoine Borensztejn*, Leigh K. Harris*, Sara Carlson, Gokhan Dalgin, Suraj Mishra, Nivedita Nivedita, Ellen M. Adams, Tiffany Barszczewski, Julie C. Dixon, Jacqueline H. Edmonds, Erik A. Ehlers, Alexandra J. Ferrante, Margaret A. Fuqua, Philip Garrison, Janani Gopalan, Benjamin W. Gregor, Maxwell J. Hedayati, Kyle N. Klein, Chantelle L. Leveille, Sean L. Meharry, Haley S. Morris, Gouthamrajan Nadarajan, Sandra A. Oluoch, Serge E. Parent, Amber Phan, Brock Roberts, Emmanuel E. Sanchez, M. Filip Sluzewski, Lev S. Snyder, Derek J. Thirstrup, John Paul Thottam, Julia R. Torvi, Gaea Turman, Matheus P. Viana, Lyndsay Wilhelm, Chamari S. Wijesooriya, Jie Yao, Julie A. Theriot, Susanne M. Rafelski & Ruwanthi N. Gunawardane
  
• Automated human induced pluripotent stem cell culture and sample preparation for 3D live-cell microscopy by Benjamin W. Gregor*, Mackenzie E. Coston*, Ellen M. Adams, Joy Arakaki, Antoine Borensztejn, Thao P. Do, Margaret A. Fuqua, Amanda Haupt, Melissa C. Hendershott, Winnie Leung, Irina A. Mueller, Aditya Nath, Angelique M. Nelson, Susanne M. Rafelski, Emmanuel E. Sanchez, Madison J. Swain-Bowden, W. Joyce Tang, Derek J. Thirstrup, Winfried Wiegraebe, Brian P. Whitney, Calysta Yan, Ruwanthi N. Gunawardane & Nathalie Gaudreault
  
• Cell states beyond transcriptomics: Integrating structural organization and gene expression in hiPSC-derived cardiomyocytes by Kaytlyn A. Gerbin*, Tanya Grancharova*, Rory M. Donovan-Maiye*, Melissa C. Hendershott*, Helen G. Anderson, Jackson M. Brown, Jianxu Chen, Stephanie Q. Dinh, Jamie L. Gehring, Gregory R. Johnson, HyeonWoo Lee, Aditya Nath, Angelique M. Nelson, M. Filip Sluzewski, Matheus P. Viana, Calysta Yan, Rebecca J. Zaunbrecher, Kimberly R. Cordes Metzler, Nathalie Gaudreault, Theo A. Knijnenburg, Susanne M. Rafelski, Julie A. Theriot & Ruwanthi N. Gunawardane
  
• Colony context and size-dependent compensation mechanisms give rise to variations in nuclear growth trajectories by Julie C. Dixon*, Christopher L. Frick*, Chantelle L. Leveille*, Philip Garrison, Peyton A. Lee, Saurabh S. Mogre, Benjamin Morris, Nivedita Nivedita, Ritvik Vasan, Jianxu Chen, Cameron L. Fraser, Clare R. Gamlin, Leigh K. Harris, Melissa C. Hendershott, Graham T. Johnson, Kyle N. Klein, Sandra A. Oluoch, Derek J. Thirstrup, M. Filip Sluzewski, Lyndsay Wilhelm, Ruian Yang, Daniel M. Toloudis, Matheus P. Viana, Julie A. Theriot & Susanne M. Rafelski
  
• Integrated intracellular organization and its variations in human iPS cells by Matheus P. Viana, Jianxu Chen*, Theo A. Knijnenburg*, Ritvik Vasan*, Calysta Yan*... Allen Institute for Cell Science... Graham T. Johnson, Ruwanthi N. Gunawardane, Nathalie Gaudreault, Julie A. Theriot & Susanne M. Rafelski
  
• Interpretable representation learning for 3D multi-piece intracellular structures using point clouds by Ritvik Vasan, Alexandra J. Ferrante, Antoine Borensztejn, Christopher L. Frick, Nathalie Gaudreault, Saurabh S. Mogre, Benjamin Morris, Guilherme G. Pires, Susanne M. Rafelski, Julie A. Theriot & Matheus P. Viana
  
• Label-free prediction of three-dimensional fluorescence images from transmitted-light microscopy by Chawin Ounkomol, Sharmishtaa Seshamani, Mary M. Maleckar, Forrest Collman & Gregory R. Johnson
  
• Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization by Brock Roberts*, Amanda Haupt*, Andrew Tucker, Tanya Grancharova, Joy Arakaki, Margaret A. Fuqua, Angelique Nelson, Caroline Hookway, Susan A. Ludmann, Irina A. Mueller, Ruian Yang, Rick Horwitz, Susanne M. Rafelski & Ruwanthi N. Gunawardane
  

Cell Painting Gallery

Tutorials

• Cell Painting wiki by Multiple Authors
  
• Center for Open Bioimage Analysis (COBA) YouTube Channel - video tutorials of CellProfiler and other softwares by Multiple Authors
  
• Image-based Profiling Handbook - for processing image-based profiling datasets using CellProfiler and pycytominer by Multiple Authors
  
• Image-based profiling introductory exercise - data and an exercise on exploring image-based profiles, including understanding the various data levels by Beth Cimini
  
• Scientific Community Image Forum - for software-oriented aspects of scientific imaging including analysis, processing, and acquisition by Multiple Authors
  

Tools & Applications

• Accelerating Drug Discovery with high-throughput Cell Painting on AWS by Chris Kaspar
  
• Deep Profiler - Morphological profiling using deep learning by Multiple Authors
  
• Distributed CellProfiler - Run encapsulated docker containers with CellProfiler in the Amazon Web Services infrastructure by Multiple Authors
  
• Image-based Profiling Recipe by Multiple Authors
  
• Pycytominer - Data processing functions for profiling perturbations by Multiple Authors
  

Publications

• A dataset of images and morphological profiles of 30 000 small-molecule treatments using the Cell Painting assay by Bray M-A, Gustafsdottir SM, Rohban MH, Singh S, Ljosa V, Sokolnicki KL, Bittker JA, Bodycombe NE, Dancik V, Hasaka TP, Hon CS, Kemp MM, Li K, Walpita D, Wawer MJ, Golub TR, Schreiber SL, Clemons PA, Shamji AF, & Carpenter AE
  
• Cell Painting predicts impact of lung cancer variants by Caicedo JC, Arevalo J, Piccioni F, Bray MA, Hartland CL, Wu X, Brooks AN, Berger AH, Boehm JS, Carpenter AE, & Singh S
  
• Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes by Bray M-A, Singh S, Han H, Davis CT, Borgeson B, Hartland C, Kost-Alimova M, Gustafsdottir SM, Gibson CC, & Carpenter AE
  
• Morphological Profiles of RNAi-Induced Gene Knockdown Are Highly Reproducible but Dominated by Seed Effects by Singh S, Wu X, Ljosa V, Bray M-A, Piccioni F, Root DE, Doench JG, Boehm JS, & Carpenter AE
  
• Multiplex Cytological Profiling Assay to Measure Diverse Cellular States by Gustafsdottir SM, Ljosa V, Sokolnicki KL, Wilson JA, Walpita D, Kemp MM, Seiler KP, Carrel HA, Golub TR, Schreiber SL, Clemons PA, Carpenter AE, and Shamji AF
  
• Systematic morphological profiling of human gene and allele function via Cell Painting by Rohban MH, Singh S, Wu X, Berthet JB, Bray M-A, Shrestha Y, Varelas X, Boehm JS, & Carpenter AE
  
• Toward performance-diverse small-molecule libraries for cell-based phenotypic screening using multiplexed high-dimensional profiling by Wawer MJ, Li K, Gustafsdottir SM, Ljosa V, BodycombeNE, Marton MA, Sokolnicki KL, Bray M-A, Kemp MM, Winchester E, Taylor B, Grant GB, Hon CSK, Duvall JR, Wilson JA, Bittker JA, Dancik V, Narayan R, Subramanian A, Winckler W, Golub TR, Carpenter AE, Shamji AF, Schreiber SL, & Clemons PA
  

Fly Brain Anatomy: FlyLight Gen1 and Split-GAL4 Imagery

Tutorials

• File Operations on AWS S3 by Rob Svirskas
  
• Scaling Neuroscience Research on AWS by Konrad Rokicki
  AWS LambdaAWS AppSyncAmazon DynamoDBAWS Step Functions
• Tutorial for neuronbridger (R API) by Alexander Bates
  
• Using Imagery on AWS S3 by Rob Svirskas
  

Tools & Applications

• Color Depth Search Fiji Plugin by Hideo Otsuna
  
• Fly Light Split-GAL4 Driver Collection by Rob Svirskas
  
• FlyLight Project Website by Geoffrey Meissner
  
• NeuronBridge by Jody Clements, Rob Svirskas, Hideo Otsuna, Cristian Goina, Konrad Rokicki
  

Publications

• A GAL4-Driver Line Resource for Drosophila Neurobiology by Arnim Jenett, Gerald M Rubin, Teri-TB Ngo, David Shepherd, Christine Murphy, Heather Dionne, Barret D Pfeiffer, Amanda Cavallaro, Donald Hall, Jennifer Jeter, Nirmala Iyer, Dona Fetter, Joanna H Hausenfluck, Hanchuan Peng, Eric T Trautman, Robert R Svirskas, Eugene W Myers, Zbigniew R Iwinski, Yoshinori Aso, Gina M DePasquale, Adrianne Enos, Phuson Hulamm, Shing Chun Benny Lam, Hsing-Hsi Li, Todd R Laverty, Fuhui Long, Lei Qu, Sean D Murphy, Konrad Rokicki, Todd Safford, Kshiti Shaw, Julie H Simpson, Allison Sowell, Susana Tae, Yang Yu, Christopher T Zugates
  
• An image resource of subdivided Drosophila GAL4-driver expression patterns for neuron-level searches by Geoffrey W Meissner, Zachary Dorman, Aljoscha Nern, Kaitlyn Forster, Theresa Gibney, Jennifer Jeter, Lauren Johnson, Yisheng He, Kelley Lee, Brian Melton, Brianna Yarbrough, Jody Clements, Cristian Goina, Hideo Otsuna, Konrad Rokicki, Robert R Svirskas, Yoshinori Aso, Gwyneth M Card, Barry J Dickson, Erica Ehrhardt, Jens Goldammer, Masayoshi Ito, Wyatt Korff, Ryo Minegishi, Shigehiro Namiki, Gerald M Rubin, Gabriella Sterne, Tanya Wolff, Oz Malkesman, FlyLight Project Team
  
• An unbiased template of the Drosophila brain and ventral nerve cord by John A Bogovic, Hideo Otsuna, Larissa Heinrich, Masayoshi Ito, Jennifer Jeter, Geoffrey Meissner, Aljoscha Nern, Jennifer Colonell, Oz Malkesman, Kei Ito, Stephan Saalfeld
  
• Color depth MIP mask search: a new tool to expedite Split-GAL4 creation by Hideo Otsuna, Masayoshi Ito, Takashi Kawase
  
• The neuronal architecture of the mushroom body provides a logic for associative learning by Yoshinori Aso, Daisuke Hattori, Yang Yu, Rebecca M Johnston, Nirmala A Iyer, Teri-TB Ngo, Heather Dionne, LF Abbott, Richard Axel, Hiromu Tanimoto, Gerald M Rubin
  

Automated Segmentation of Intracellular Substructures in Electron Microscopy (ASEM) on AWS

Tutorials

• Data layout and how to view by kirchhausenlab
  
• How to use models by kirchhausenlab
  

Tools & Applications

• ASEM Colab Notebook (Interactive Demo) by Patrick Stock
  
• TK Lab Data Explorer by Patrick Stock
  

Publications

• Deep neural network automated segmentation of cellular structures in volume electron microscopy by Benjamin Gallusser, Giorgio Maltese, Giuseppe Di Caprio, Tegy John Vadakkan, Anwesha Sanyal, Elliott Somerville, Mihir Sahasrabudhe, Justin O’Connor, Martin Weigert, Tom Kirchhausen
  

SPARC: Datasets bridging the body and the brain

Tutorials

• Download public data, scaffolds and run computations by The SPARC Data and Resource Center
  
• Downloading large scale SPARC datasets by The SPARC Data and Resource Center
  
• Using sparc.client for data movement in SPARC by The SPARC Data and Resource Center
  

Tools & Applications

• OSPARC by Esra Neufeld
  
• SODA Tool by Bhavesh Patel
  
• The Pennsieve Data Management Platform by Joost Wagenaar
  
• The SPARC Portal by Peter Hunter, Maryann Martone, Esra Neufeld, Joost Wagenaar
  

Publications

• The SPARC DRC: Building a Resource for the Autonomic Nervous System Community by Osanlouy M, Bandrowski A, de Bono B, Brooks D, Cassara A, Christie R, Ebrahimi N, Gillespie T, Grethe J, Guercio L, Heal M, Lin M, Kuster N, Martone M, Neufeld E, Nickerson D, Soltani E, Tappan S, Wagenaar J, Zhuang K, Hunter P
  

International Skin Imaging Collaboration (ISIC) Archive

Tutorials

• ISIC Archive Data Dictionary by International Skin Imaging Collaboration (ISIC)
  

Tools & Applications

• ISIC Archive Gallery by International Skin Imaging Collaboration (ISIC)
  
• isic-cli - The official command line tool for interacting with the ISIC Archive by International Skin Imaging Collaboration (ISIC)
  

Publications

• A patient-centric dataset of images and metadata for identifying melanomas using clinical context by Rotemberg V, Kurtansky N, Betz-Stablein B, Caffery L, Chousakos E, Codella N, et al
  
• Human surface anatomy terminology for dermatology: a Delphi consensus from the International Skin Imaging Collaboration by Navarrete-Dechent C, Liopyris K, Molenda M, Braun R, Curiel-Lewandrowski C, Dusza S, et al
  
• International Skin Imaging Collaboration - Designated Diagnoses (ISIC-DX): Consensus terminology for lesion diagnostic labeling by Scope A, Liopyris K, Weber J, Barnhill R, Braun R, Curiel-Lewandrowski C, et al
  
• The SLICE-3D dataset: 400,000 skin lesion image crops extracted from 3D TBP for skin cancer detection by Kurtansky N, D'Alessandro B, Gillis M, Betz-Stablein B, Cerminara S, Garcia R, et al
  

Mouse Brain Anatomy: MouseLight Imagery

Tools & Applications

• HortaCloud by David Schauder, Donald J. Olbris, Jody Clements, Cristian Goina, Robert R. Svirskas, Konrad Rokicki
  
• MouseLight NeuronBrowser by Tiago A. Ferreira, Jayaram Chandrashekar
  
• MouseLight Project Website by Tiago A. Ferreira, Jayaram Chandrashekar
  

Publications

• Reconstruction of 1,000 Projection Neurons Reveals New Cell Types and Organization of Long-Range Connectivity in the Mouse Brain by Johan Winnubst, Erhan Bas, Tiago A. Ferreira, Zhuhao Wu, Michael N. Economo, Patrick Edson, Ben J. Arthur, Christopher Bruns, Konrad Rokicki, David Schauder, Donald J. Olbris, Sean D. Murphy, David G. Ackerman, Cameron Arshadi, Perry Baldwin, Regina Blake, Ahmad Elsayed, Mashtura Hasan, Daniel Ramirez, Bruno Dos Santos, Monet Weldon, Amina Zafar, Joshua T. Dudman, Charles R. Gerfen, Adam W. Hantman, Wyatt Korff, Scott M. Sternson, Nelson Spruston, Karel Svoboda, Jayaram Chandrashekar
  

OpenCell on AWS

Tools & Applications

• cytoself (an unsupervised ML model to quantify localization patterns) by Hirofumi Kobayashi, Keith C. Cheveralls, Manuel D. Leonetti, Loic A. Royer
  
• OpenCell web portal by OpenCell team
  

Publications

• OpenCell: proteome-scale endogenous tagging enables the cartography of human cellular organization by Nathan H. Cho, Keith C. Cheveralls, Andreas-David Brunner, Kibeom Kim, André C. Michaelis, Preethi Raghavan, et al.
  
• Self-Supervised Deep-Learning Encodes High-Resolution Features of Protein Subcellular Localization by Hirofumi Kobayashi, Keith C. Cheveralls, Manuel D. Leonetti, Loic A. Royer
  

CZ Grand Challenges - Imaging MIT Licensed data and models

Tutorials

• Documentation for CELL-Diff by CZI
  
• Documentation for SubCell by CZI
  
• Quickstart Tutorial for CELL-Diff by CZI
  
• Quickstart Tutorial for SubCell by CZI
  

Publications

• CELL-Diff: Unified diffusion modeling for protein sequences and microscopy images by Zheng Dihan, Bo Huang
  
• SubCell: Vision foundation models for microscopy capture single-cell biology by Ankit Gupta, Zoe Wefers, Konstantin Kahnert, Jan N Hansen, William D. Leineweber, Anthony Cesnik, Dan Lu, Ulrika Axelsson, Frederic Ballllosera Navarro, Theofanis Karaletsos, Emma Lundberg
  

National Cancer Institute Imaging Data Commons (IDC) Collections

Tutorials

• IDC "Getting started" tutorial series by Imaging Data Commons team
  

Tools & Applications

• IDC Portal by Imaging Data Commons team
  

Publications

• NCI Imaging Data Commons by Fedorov A, Longabaugh W, Pot D, Clunie D, Pieper S, Aerts H, et al.
  

Biological and Physical Sciences (BPS) Microscopy Benchmark Training Dataset

Publications

• Dose, LET and Strain Dependence of Radiation-Induced 53BP1 Foci in 15 Mouse Strains Ex Vivo Introducing Novel DNA Damage Metrics by Sébastien Penninckx, Egle Cekanaviciute, Charlotte Degorre, Elodie Guiet, Louise Viger, Stéphane Lucasb, Sylvain V. Costes
  
• NASA SMD AI Workshop Report by SMD Artificial Intelligence (AI) Initiative
  

Brain/MINDS Marmoset Connectivity Resource on AWS

Tutorials

• Explorer Tutorials by Henrik Skibbe
  

Tools & Applications

• BMCR website by Henrik Skibbe
  
• Marmoset PFC connectome by Akiya Watakabe, Henrik Skibbe and Tetsuo Yamamori
  

Publications

• Local and long-distance organization of prefrontal cortex circuits in the marmoset brain. by Watakabe A, Skibbe H, Nakae K, Abe H, Ichinohe N, Rachmadi MF, Wang J, Takaji M, Mizukami H, Woodward A, Gong R, Hata J, Van Essen DC, Okano H, Ishii S, Yamamori T.
  
• The Brain/MINDS Marmoset Connectivity Resource - An open-access platform for cellular-level tracing and tractography in the primate brain by H. Skibbe, M.F. Rachmadi, K. Nakae, C. E. Gutierrez, J. Hata, H. Tsukada, C. Poon, K. Doya, P. Majka, M. G. P. Rosa, M. Schlachter, H. Okano, T. Yamamori, S. Ishii, M. Reisert, A. Watakabe.
  

Cell Painting Image Collection

Tools & Applications

• Accelerating Drug Discovery with high-throughput Cell Painting on AWS by Chris Kaspar
  

Publications

• Example submission for the 2018 CytoData Hackathon (in R and Python) by Juan Caicedo, Tim Becker
  

Seattle Alzheimer's Disease Brain Cell Atlas (SEA-AD)

Tools & Applications

• Seattle Alzheimer’s Disease Brain Cell Atlas by Lein, E et al.
  

CZ Grand Challenges - Imaging BSD licensed data and models

Tutorials

• Documentation for Cytoland by CZI
  
• Quickstart Tutorial for Cytoland by CZI
  

Publications

• Cytoland: robust virtual staining of landmark organelles by Liu, Hirata-Miyasaki, et al.
  

DHARANI Developing Human-Brain Atlas

Tutorials

• Accessing DHARANI data by SGBC-IITM
  

Tools & Applications

• DHARANI Web Portal by SGBC-IITM
  

Publications

• DHARANI- A 3D Developing Human-brain Atlas Resource to Advance Neuroscience Internationally -- Integrated Multimodal Imaging and High-resolution Histology of the second trimester by Verma, et al.